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Image Search Results
Journal: Frontiers in Immunology
Article Title: The phytohormone abscisic acid enhances remyelination in mouse models of multiple sclerosis
doi: 10.3389/fimmu.2024.1500697
Figure Lengend Snippet: ABA promotes lipid droplet load in macrophages in vitro. (A) Schematic representation showing the experimental set-up: mouse BMDMs were left untreated or treated with 100µg/ml myelin for 24 hours (Mye 24h ) or 72 hours (Mye 72h ). Myelin exposure was performed in the presence or absence of ABA. Created with biorender.com. (B) Representative images of ORO staining of BMDMs exposed to vehicle (PBS) or ABA and treated with myelin for 24 or 72 hours. Scale bars, 50 µm. (C) Quantification of lipid load (defined as percent ORO+ area of total cell area)(n = 3). (D) Mean fluorescence intensity of BODIPY in BMDMs (n = 5) exposed to vehicle or ABA and treated with myelin for 24 or 72 hours, as measured by flow cytometry. Data is represented as relative lipid droplet load compared to vehicle. (E) Internalization of pHrodo™-labelled myelin by BMDMs exposed to vehicle or ABA for 24 hours. Data are measured by flow cytometry and depicted relative to the vehicle treated group. (F) Quantification of total cholesterol (TC), free cholesterol (FC), and esterified cholesterol (EC) in BMDMs (n = 6) exposed to vehicle or ABA and treated with myelin for 0, 24 or 72 hours. (G-I) mRNA expression of Igf1, Tgfβ and Tnf α in BMDMs (n = 4). Each dot represents one well. Data are represented as mean ± SEM and statistically analyzed using a one-way ANOVA with correction for multiple testing or Student’s t-test. *p<0.05, **p<0.01.
Article Snippet: Chemokines and cytokines were measured in plasma using a Bio-Plex assay for
Techniques: In Vitro, Staining, Fluorescence, Flow Cytometry, Expressing